Abstract A112: Molecular variants of E7/ HPV58 in women with normal citology from the Colombian cohort. Preliminary results

Abstract

Abstract A112 Introduction Human papillomavirus type 58 (HPV 58) is the second viral type more prevalent in women with normal cytology from the Colombian population. Also this type is highly prevalent in women with high grade squamous intraepithelial cervical lesions (HGSIL) and in women with invasive cervical cancer worldwide. There are few studies of E7/HPV 58 variants, however some of them have shown an association of variants presence with a higher risk of HGSIL and cervical cancer in Asian populations. In Colombia there are not studies of presence and persistence of E7/HPV58 variants. Objective To identify molecular variants of E7/HPV58 in cervical scrapes of women with normal cytology that belongs to the base line of the Bogotá, Colombia Cohort. Materials and Methods 1845 cervical scrapes of women with normal cytology that belong to the base line of the Bogotá, Colombia cohort were analysed. HPV detection was done using the GP5+/GP6+ primers and 37 different HPV types were typed using a PCR-RLB assay. The E7 region of samples HPV 58 positives was amplified using the E7P1- E7P2 primers that amplify a fragment of 316 bp (561 to 877 nucleotides). E7/HPV 58 variants were detected using automated direct sequencing. The reference sequence of HPV 58 was used to compare sequences. Results 34 samples were positive for HPV 58 (1.84%). Until this moment 13 samples have been analysed for E7/HPV 58 variants. 12 of these samples have the A694/G744/A761 variant (94.7%), one sample have the A599/A694/G744/A761 variant (5,5%), and none of the samples had the reference sequence of HPV58. Conclusions Two variants have been identified in the samples analysed: 1 previously identified in Asian population and 1 new variant (A599/A694/G744/A761). There are few diversity of E7/ HPV58 variants in the analysed samples. The cohort analysis will give important information about the role of these variants in the persistence of HPV 58 infection and in the development of cervical intraepithelial lesions. Citation Information: Cancer Prev Res 2008;1(7 Suppl):A112.