Abstract A10: LncRNA MIR222HG transcribed from the miR-221/222 promoter are associated with the development of castration resistant prostate cancer

Abstract

Abstract We previously found that microRNA-221 and 222 play a crucial role in regulating the AR function during the castration resistant prostate cancer (CRPC) development. We further investigated mechanisms involved in the differential expression of miR-221/222 in prostate cancer cell lines. Based on transcriptome and histone modification data, we recognized a putative promoter region located at 23.3 kb upstream of the miR-221/222. By further characterizing transcriptional events occurred in this promoter region, we identified a two- exonic, polyadenylated long non-coding RNA (lncRNA), MIR222HG. Forced over-expression MIR222HG significantly increased androgen-independent cell growth and repressed DHT induced PSA expression in LNCaP cells, a hallmark of CRPC phenotype. Expression level of MIR222HG is significantly associated with human prostate cancer progression from localized hormone sensitive prostate cancer (HSPC) to CRPC. Taken together, lncRNA MIR222HG, which is co-transcribed from miR-221/222 promoter region, mediated AR transcriptional regulation and may involved in transformation process from HSPC to CRPC. Further study on MIR222HG may help understand the CRPC development processes. Citation Format: Fangfang Qu, Tong Sun, Xiaodong Wang, Gwo-Shu Mary Lee, Philip W. Kantoff. LncRNA MIR222HG transcribed from the miR-221/222 promoter are associated with the development of castration resistant prostate cancer. [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer: Mechanisms to Medicines ; 2015 Dec 4-7; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2016;76(6 Suppl):Abstract nr A10.