Abstract A097: Regulation of immunophenotype modulation of monocytes-macrophages from M1 to M2 by prostate cancer cell culture supernatant via the transcription factor STAT3

Abstract

Abstract Background: The transcription factor STAT3 has a prominent innate immunity effect on cancer progression. We determined the regulation of STAT3 in the immunophenotype modulation of macrophages from M1 to M2 induced by the cell culture supernatant of the prostate cancer line PC3. Methods: Monocyte-macrophages from healthy donors were cultured in the supernatant of PC3 cells, membrane proteins and intracytoplasmic and phosphorylated STAT3 were measured using flow cytometry, and cytokines and growth factors were studied using luminescence. Cytotoxicity and nitric oxide were evaluated via colorimetric assays. Results: The supernatant of prostate tumor PC3 cells effectively induced macrophages toward an M2 profile, and the expression of phosphorylated STAT3 in the monocytes-macrophages notably increased. In the group of monocytes-macrophages treated with an STAT3 inhibitor, the macrophages were induced towards an M1 phenotype. Conclusions: In this study, we show that the secretion profile of PC3 prostate cancer cells induces a change in macrophage phenotype from M1 to M2, and this phenomenon is related to phosphorylation of the transcription factor STAT3. Citation Format: Raul Solis, Alejandro Bravo, Jr., Georgina Hernandez, Alejandro Bravo. Regulation of immunophenotype modulation of monocytes-macrophages from M1 to M2 by prostate cancer cell culture supernatant via the transcription factor STAT3 [abstract]. In: Proceedings of the AACR Special Conference: Prostate Cancer: Advances in Basic, Translational, and Clinical Research; 2017 Dec 2-5; Orlando, Florida. Philadelphia (PA): AACR; Cancer Res 2018;78(16 Suppl):Abstract nr A097.