Abstract 995: The role of intracellular ATP in regulating glycolysis, HIF1α and drug resistance of cancer cells

Abstract

Abstract Cancer is also considered to be a metabolic disease due to a shift of glucose metabolism from oxidative phosphorylation to anaerobic glycolysis. This study is to understand the effect of intracellular ATP of cancer cells on various metastatsis related phenotypes, including glycolysis, hypoxia stability and drug resistance. In this study, we delivered ATP directly into cancer cells by embedding ATP into liposomes. Normal cells and its corresponding tumor cells were measured for their whole cell ATP content. Glycolysis was evaluated by measurement of glucose consumption and lactate accumulation of cell culture media. Cell cycle was analyzed using flow cytometry after drug treatment and delivery of ATP. HIF-1α expression level was measured by western blot after delivery of ATP. In summary, normal cells have overall higher ATP content than tumor cells. After delivery of ATP, cells consumed less glucose and produced less lactate acid, which indicated a decrease in glycolysis. ATP liposomes prevented MDA-MB-231 cells from G2 arrest by adriamycin. HIF1a was down regulated after delivery of ATP. Taken together, ATP content is lower in cancer cells than normal cells, and intracellular ATP plays a role in regulating glycolysis, HIF-1α stability and drug resistance of cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 995. doi:10.1158/1538-7445.AM2011-995