Abstract 987: A novel cell cycle-associated lncRNA, HOXA11-AS, is transcribed from 5-prime end of HOXA transcript and a biomarker of progression in glioma

Abstract

Abstract The comprehensive lncRNA expression signature in glioma remains fully unknown. We performed a high throughput microarray to detect ncRNA expression profile in 225 human glioma tissues. One novel lncRNA, HOXA11-AS with unknown function, is the antisense transcript of HOX11 gene. The results showed that HOXA11-AS was closely associated with glioma grade and poor prognosis. Multivariate cox regression analysis revealed that HOXA11-AS was an independent prognostic factor in glioblastoma multiforme patients and its expression was correlated with glioma molecular subtype of the Cancer Genome Atlas. Gene set enrichment analysis indicated that gene sets most correlated with HOXA11-AS expression involved in cell cycle progression. Overexpression of HOXA11-AS transcript promoted cell proliferation in vitro while knockdown of HOXA11-AS expression manifested a repressive function during these cellular processes via regulating cell cycle progress. The growth promoting and inhibiting effect of HOXA11-AS was also demonstrated in a xenograft mouse model. For the first time, our data confirms that HOXA11-AS is an important long non-coding RNA that primarily serves as a prognostic factor for glioma patient survival, a biomarker for identifying glioma molecular subtypes, and therapeutic target for glioma patient. Citation Format: Qixue Wang, Junxia Zhang, Yanwei Liu, Junhju Zhou, Chunsheng Kang, Lei Han. A novel cell cycle-associated lncRNA, HOXA11-AS, is transcribed from 5-prime end of HOXA transcript and a biomarker of progression in glioma. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 987.