Abstract: 9.50 The Assessment Of The Protective Effect Of Lactobacillus Plantarum From Methicillin-Resistant Staphylococcus Aureus Infections In An Experimental Burn Model

Abstract

INTRODUCTION: Methicillin-resistant Staphylococcus aureus is one of the most common microorganisms isolated in graft lysis and septic patients with extensive burns. The main objective of this study is to determine and quantitatively measure the protective and/or therapeutic effect of Lactobacillus plantarum application on the burn wound before and after the onset of Methicillin-resistant Staphylococcus aureus infection. MATERIALS AND METHODS: For this reason third degree scald burn lesions affecting 10% of body surface area were formed on the back of five groups of rats. There were seven rats in each group with a total number of thirty-five. First group designed as control group and no intervention made after the burn. In the second group, Lactobacillus plantarum was applied right after the burn and then Methicillin-resistant Staphylococcus aureus was inoculated. In the third group, Methicillin-resistant Staphylococcus aureus was applied right after the burn and then Lactobacillus plantarum was inoculated. The other two groups were designed as controls of Lactobacillus plantarum and Methicillin-resistant Staphylococcus aureus. RESULTS: On the fifth and tenth days bacterial loads and compositions were assessed by colony counts in tissue biopsies and wound swabs. In the second group in which Lactobacillus plantarum was applied before Methicillin-resistant Staphylococcus aureus infection, Methicillin-resistant Staphylococcus aureus colony counts were found to be significantly lower compared to the other groups (p<0.05). In the third group in which Lactobacillus plantarum was applied after Methicillin-resistant Staphylococcus aureus infection, Methicillin-resistant Staphylococcus aureus colony counts were not found to be significantly different compared to control groups (p>0.05). As a result Lactobacillus plantarum has shown to have a protective role in non-infected burn wounds when applied before Methicillin-resistant Staphylococcus aureus infection but a therapeutic effect of Lactobacillus plantarum was not demonstrated. CONCLUSIONS: Lactobacillus plantarum is thought to have a promising role in prevention and treatment of burn wound infections.