Abstract 9253: Proteomic and Functional Assessment of High Density Lipoprotein Subclasses in Acute Coronary Syndromes Subjects as Well as Control Subjects

Abstract

Objectives: To explicit whether the functions of HDL subclasses (HDL 2 and HDL 3 ) are impaired in subjects with acute coronary syndromes (ACS). And on top of that, we confirm that alterations in HDL subclasses proteomics may exert an instrumental effect on the occurence of dysfunctional HDL subclasses. Methods: We measured HDL subclasses inflammatory index (HII), paraoxonase (PON) activity and LOOH levels in 30 patients with ACS compared with 30 healthy subjects. Additionally, proteomics was used to monitor changes in the HDL subclasses proteome between controls and ACS subjects. HDL subfractions were isolated by ultracentrifugation and the proteins were separated with 2-D gel electrophoresis and identified with mass spectrometry. Results: Subjects with ACS had higher HII and HDL-LOOH levels compared with controls; Paraoxonase activity in ACS group was significantly lower than that of control group (Table 1). Using proteome analysis, we demonstrated that compared with control group, HDL 3 from subjects with ACS was selectively enriched in 8 proteins (apoA-I, apoA-IV, apoE, apoL1, PON, alpha-1B-glycoprotein, amyloid P-component, fibrinogen gamma chain), and meanwhile ras-related protein Rab-7b was decreased in HDL 3 . Moreover, HDL 2 from ACS group was selectively lower in 10 proteins (apoE, PON, apoA-IV, apoL1, haptoglobin, hemopexin, serotransferrin, complement factorB, fibrinogen gamma chain,ras-related protein Rab-7b); but 2 proteins were increased in HDL 2 (amyloid P-component, alpha-1 antitrypsin). Conclusions: HDL subclasses have less anti-inflammatory and anti-oxidant capacities in ACS patients compared with controls. Moreover, the study also suggest that composition alteration of HDL subfractions may play a pivotal role in the progress of functional HDL subclasses shift to dysfunctional HDL subclasses. Furthermore, this research reveals new proteins in HDL 2 and HDL 3 ; serum amyloid P-component and ras-related protein Rab-7b.