Abstract 902: RNA-Seq profiling of silibinin effects on bladder cancer cells

Abstract

Abstract Silibinin is the major active constituent of silymarin, an extract of the milk thistle seeds, containing a mixture of flavonolignans such as silibinin and isosilibinin. Silibinin has been shown to have significant anti-cancer effects in a variety of cancer types, including bladder, kidney, prostate, skin, breast, lung and colon cancer. Silibinin (10 μM) significantly suppressed proliferation and invasion of T24 and UM-UC-3 bladder cancer cells. Western blot analysis showed that silibinin significantly reduces expression of vimentin and slug in these bladder cells. Vimentin is overexpressed in various cancers and is known to promote tumor growth, invasion, and poor prognosis. Slug, a transcriptional repressor which mediates epithelial to mesenchymal transition (EMT), has been implicated in tumor metastasis. These results indicate that silibinin inhibits proliferation and invasion by suppressing vimentin and slug in bladder cancer cells. We studed the anti-cancer effects of silibinin in bladder cancer cells using RNA-Seq, a transcriptome profiling technique that uses deep-sequencing to quantitatively investigate the global behavior of transcriptomes. We will discuss the RNA-Seq profiling of the anti-cancer effects of silibinin in bladder cancer cells, including the vimentin and slug networks. Citation Format: Soichiro Yamamura, Yozo Mitsui, Nathan Bucay, Sharanjot Saini, Shahana Majid, Guoren Deng, Varahram Shahryary, Rajvir Dahiya, Yuichiro Tanaka. RNA-Seq profiling of silibinin effects on bladder cancer cells. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 902. doi:10.1158/1538-7445.AM2015-902