Abstract 747: Characteristic molecular signature of pericardial effusion identifies malignant cancer in pericardial disorder patients

Abstract

Abstract Pericardial effusion (PE) can develop in patients with virtually any condition that affects the pericardium, including acute pericarditis and a variety of systemic disorders. PE is also common in cancer patients, and that may develop by several different mechanisms by direct or metastatic spread of the primary process or as a complication of antineoplastic therapies. Thus, definite differentiation of malignant PE and rapid diagnosis are of particular therapeutic and prognostic importance. We aim to determine etiology-based characteristic molecular changes of PE in pericardial disorder patients, and to identify novel molecular signature for early detection of cancer patients with pericardial disorder. We obtained pericardial fluids from the pericardial disorders including cancer patients. Whole genomic transcriptome scan resulted in 1,639 outlier genes as cancer-specific PE molecular signature. Further analysis showed that a cancer-specific molecular signature exists and discerns cancer patients with non-cancer patients, and that also suggested 191 gene elements as stringent classifier between cancer vs non-cancer (Welch’s t test, p < 0.05 and Ven diagrammatic analysis). Gene set enrichment analysis (GSEA) with additional computational analysis of 1,639 cancer-specific PE molecular signature identified CD24, SDC1, and ST14 as strong molecular markers for identifying cancer in PE patients. In conclusion, whole transcriptomic analysis of pericardial fluids of pericardial disorder patients suggest that etiology-specific molecular signatures can discriminate different patterns of PE, and that they provide useful information for mechanistic studies that may lead to a better understanding of the molecular basis of PE pathology. Keywords: pericardial fluid, differential gene expression, diagnostic markers, CD24, SDC1, ST14 Citation Format: Suk Woo Nam, Sang Yean Kim, Hyung Seok Kim, Hee Doo Yang, Min Jeong Na. Characteristic molecular signature of pericardial effusion identifies malignant cancer in pericardial disorder patients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 747.