Abstract 6000: eIF3A regulate the Warburg effect via ENO1 in cervical cancer cells

Abstract

Abstract Eukaryotic translation initiation factor 3A (eIF3A) is one of the core subunits of the translation initiation complex eIF3. eIF3A is critical for ribosomal subunit joining and recruiting mRNA to the ribosome, as well as for the regulation of various gene products. However, the function of eif3A have not been reported in cervical cancer (CC). Here we reported that Eif3A protein was overexpressed in CC tissues and the higher expression of Eif3A predicted a worse prognosis. Further experiments showed that eIF3A knockdown inhibited CC cells' proliferation and migration and promoted apoptosis in vitro and in vivo. eIF3A silencing accelerated acidification of the culture medium of CC cells. Seahorse results showed that the decreased expression of eIF3A significantly reduced glycolysis flux in CC cells. Mass spectrometry suggested that eIF3A knockdown could significantly repressed the expression of ENO1, which is a famous glycolytic protein. Western blot assay indicated that eIF3A silencing significant decreased the expression of ENO1. Therefore, our results indicated that eIF3A could enhance the warburg effect in cervical cancer by regulating ENO1. Citation Format: Pengfei Xu, Dake Li. eIF3A regulate the Warburg effect via ENO1 in cervical cancer cells [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 6000.