Abstract 5668: An experimental study of folic acid coupled nano-paclitaxel for targeted treatment of ovarian cancer.

Abstract

Abstract To study the effect of folic acid coupled nano-paclitaxel in vivo and in vitro targeted treatment of ovarian cancer and preliminarily elucidate its therapeutical mechanism. Methods: The folic acid coupled nano-paclitaxel drug were prepared by the co-incubation method; The folic acid receptor-positive ovarian cancer cell lines were screened by immunohistochemical staining method; the targeting effect of the drug on SKOV3 cells were assessed qualitatively by fluorescence microscopy and quantitatively by flow cytometry (FCM). The killing effect of the drug on SKOV3 cells and SKOV3/TAX cells were monitored in vitro by plotting cell growth curve, the MTT method, FCM measurement, and electron microscopy; Tumor-burdened nude mice were administered with the drug by intraperitoneal and iv injection, the amounts of tumors were compared. The tumor killing effect of the drug in treating celiac metastases were examined qualitatively by electron microscopy and quantitatively by FCM. Results: (1) The particle size and encapsulation efficiency of folic acid coupled nano-paclitaxel drugs were (140.5±10.3)nm and 97% respectively; (2) Both SKOV3 cells and SKOV3/TAX cells were folic acid receptor positive; (3) Folic acid coupled nano-paclitaxel drugs demonstrated targeted killing effect on SKOV3 cells by the mediation of the folic acid receptor. The intracellular drug concentration gradually increases along with the incubation time. (4)The effects of folic acid coupled nano-paclitaxel drugs on SKOV3 cells and SKOV3/TAX cells were significantly better than the nano-paclitaxel, but the effects was similar when the concentrations were 0.2 and 2 μg/ml. The resistance index of SKOV3 / TAX cells was decreased in the course of treatment; (5) The cell apoptosis rates of the three groups after treatment were 84.63% in abdominal group,35.23% in veinal group,and 10.12% in control group respectively (p < 0.01). The apoptotic necrosis in tumor issues of abdominal group was quite obviously. But the tumor-burdened quantities in three groups were 3.38±1.77, 4.50±2.20, and 3.86±1.95 respectively. there were no statistical significance (P > 0.05). Conclusions: Folic acid coupled nano-paclitaxel drugs have better therapeutical effects in treating both drug-sensitive and drug-resistant ovarian cancer lines. The drugs administered through intraperitoneal injection showed better results than via iv injection. Citation Format: Hongxia Li. An experimental study of folic acid coupled nano-paclitaxel for targeted treatment of ovarian cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5668. doi:10.1158/1538-7445.AM2013-5668 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.