Abstract 5525: Antitumor effect of Realgar in combination with chemotherapy drugs on human gastric cancer cells

Abstract

Abstract Background: Realgar, a Chinese traditional medicine, of which the main component is arsenic sulfide (As4A4), has potentially anti-tumor property in various cancers. We aimed to study the antitumor effect of Realgar in combination with chemotherapy drugs on human gastric cancer cells. Methods: Human gastric cancer AGS cells were treated with Realgar in combination with Oxaliplatin, Irinotecan, Cisplatin, 5-FU and docetaxol with escalating dose level. Antitumor effect was measured by 24,48-hr growth inhibition by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell morphology was observed under microscope. Results: Realgar in combination with chemotherapy drugs exhibited a dose-dependent and time-dependent cytotoxic effect on AGS cells. The combination of Realgar and Irinotecan or Cisplatin showed the most potent synergistic effect. Conclusions: Realgar combined with chemotherapy drugs such as Irinotecan or Cisplatin has potent antitumor effect on human gastric cancer cells. Further mechanistic and animal studies are being performed. Citation Format: Lian Zhang, Sungkyoung Kim, Wenping Ding, Leizhen Zheng, Li Zhang, Yingying Tong, Siyu Chen. Antitumor effect of Realgar in combination with chemotherapy drugs on human gastric cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5525. doi:10.1158/1538-7445.AM2014-5525