Abstract 5457: Chlorinated drinking water alters carcinogenesis in a mice model of colon cancer

Abstract

Abstract Purpose: The gastrointestinal tract is constantly exposed to vast numbers of commensal bacteria and inflammatory product. The diet and enteric environment has been implicated in the pathogenesis of enterocolitis and colon cancer. Probiotic Lactbacilli modify the enteric microflora and are thought to have a beneficial effect on enterocolitis, eventually on colon cancer. We conducted this study to evaluate the effects of chlorinated drinking water which was used in general institutions. Experimental design: Mouse stains carrying colon epithelium-preferential Apc mutation (CPC;Apc mice), that show sporadic colorectal adenomas and carcinomas are used in this study. Nine of Eighteen CPC;Apc mice were consumed chlorinated drinking water (chlorinate concentrations of 10.0 mg/L) and 9 consumed tap water (chlorinate concentrations of 0.7 mg/L) for 25 week. Body weight was measured weekly. Faecal microbial analysis was performed. At sacrifice, the small and large bowel were histologically assessed. Results: Chlorinated drinking water strongly prevented intestinal tumorigenesis in terms of polyps formation in distal portion of small intestine. However, in colon we observed an increase in numbers and volumes of tumors. Body weight gain was more favorable by chlorine treated. Conclusions: These results show for the first time the efficacy and associated mechanisms of chlorinated drinking water feeding against spontaneous intestinal tumorigenesis in the CPC;Apc mice suggesting its chemopreventive potential against intestinal cancers. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5457. doi:1538-7445.AM2012-5457