Abstract 5414: The correlation between the expression of microRNA hsa-miR-101 and the prognosis of the patient with hepatocellular carcinoma

Abstract

Abstract Aim: MicroRNAs (miRNA) are small RNAs that regulate gene expression at the post-transcriptional level, and they play a various role in cancer cell such as proliferation, migration and metastasis. Our previous study identified forty-nine miRNAs that are differentially expressed by comparing 26 pair samples of hepatocellular carcinoma (HCC) tumor and adjacent liver tissue using next-generation sequencer system. One of them, hsa-miR-101 is chosen in this validation study. The hsa-miR-101 expression in HCC tumor samples and its adjacent liver tissues were measured by real-time PCR, and were examined the correlation between the miRNA expression and clinical data. Patients and Methods: Forty-three patients diagnosed as HCC had undergone surgical removal from 1991 to 1997 at the Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Japan. The formalin-fixed paraffin embedded samples were prepared using the standard protocol. The paraffin blocks were cut into 10 micrometer sections, and the tumor and its adjacent non-tumor liver tissue were collected using laser-captured microdissection and total RNA including miRNA fraction were isolated. After synthesized cDNA using miRNA specific primer, real-time PCR was performed using miRNA specific primer/probe. Results: The expression of hsa-miR-101 in tumor tissue was downregulated compared to adjacent liver tissue (P=0.003). No significant differences were obtained in survival time between higher or lower intratumoral expression level (P=0.8039). The patients were divided into two groups, higher/lower hsa-miR-101 expression in the tumor or in the adjacent liver tissue, the group of the higher expression in adjacent liver tissue and the lower in tumor was longer survival time compared to the other groups (P=0.0184). Conclusion: The hsa-miR-101 expression may be related to tumorigenesis, and both the expression in the tumor and in the adjacent liver tissue may be potential prognostic factor for HCC patients. Citation Format: Go Nakajima, Kazuhiko Hayashi. The correlation between the expression of microRNA hsa-miR-101 and the prognosis of the patient with hepatocellular carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5414.