Abstract 5380: Detection of OvCa1 in human malignancies.

Abstract

Abstract Immunohistochemistry biomarkers are currently being developed to target specific proteins found in cancer cells, which could contribute to a better understanding of specific types of cancers and provide information to develop better treatments for cancer patients. The biomarker and putative tumor suppressor, OvCa1, has a function that is not well characterized. Due to a lack of staining reagents, we developed monoclonal antibodies of OvCa1 to examine multiple human malignancies. The goal of this project was to gain a better understanding of OvCa1 and its role in cancer. Primary cancers with different histological grades as well as metastatic lesions were examined with the monoclonal antibodies. Ovarian cancer tissue samples from the Indiana University Simon Cancer Center Tissue Bank were used for this study. The samples were fixed in neutral buffered formalin and processed into paraffin blocks. The tissues were cut using a microtome and placed on slides. Staining of the OvCa1 antibody was completed using immunohistochemistry (IHC) techniques. Thirty-one samples were evaluated including low grade tumors, medium grade tumors, high grade tumors, and metastatic ovarian carcinomas. All cases revealed a range of staining intensity with the OvCa1 antibody. The results indicated that OvCa1 for the high grade, stage 3/4, ovarian carcinomas had the highest staining intensity. Medium grade tumors had less OvCa1 expression. The metastatic tumors had less staining than any of the other three grades. Immunostaining was observed primarily in the cytoplasm and nucleus of the tumor cells. In addition, we evaluated approximately 20 tumors from various different organs. These included prostate, breast, spleen, lung, colon, stomach, and kidney tumors, which were positive for immunostaining with the OvCa1 antibody. In summary, the results indicated that all histological grades expressed the biomarker, OvCa1, and the staining intensity was highest in the high grade, stage 3/4, tumors. Our preliminary studies demonstrated a further need to delineate OvCa1 as a potential biomarker, which could be used for early detection and diagnosis of ovarian cancer. Citation Format: Jessica L. Jackson, Kristen A. Grothaus, Connie J. Temm, Lindsey D. Mayo, George E. Sanduksy. Detection of OvCa1 in human malignancies. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5380. doi:10.1158/1538-7445.AM2013-5380