Abstract
Abstract Flow cytometry and mass spectrometry are widely used analytical tools in cancer research. Flow cytometry is known for cell counting, cell sorting and cancer biomarker discovery. Mass spectrometry is unparalleled in its ability to selectively detect and quantify target analytes at the molecular level. The combination of flow cytometry to isolate specific cell subtypes from biological fluids such as blood and mass spectrometry to quantify proteins contained in the selected cells provides a new approach to studying protein expression in specific cell subtypes. We have used this combination to investigate the normal levels of CD47 and SIRPA proteins in CB8+ T-cells, CD4+ T-cells, CD14+ monocytes, CD33+ myeloid cells and CD56+ NK cells isolated from blood specimens as a first step toward better understanding of how the CD47/SIRPA protein levels in these cells are affected by cancer and cancer treatment. Citation Format: Carmen Fernandez-Metzler, Renold Capocasale. LC-MS/MS quantification of proteins CD47 and SIRPA in cell subtypes selected by flow cytometry from blood. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5318. doi:10.1158/1538-7445.AM2014-5318
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
