Abstract 5316: Significant changes in D2-like dopamine gene receptors expression associated with non- small -cell lung cancer: A case control study.

Abstract

Abstract Introduction : Lung cancer is the leading cause of cancer-related death and a major health problem worldwide. Approximately 75% of lung tumors are non - small - cell lung cancer (NSCLC) .The alterations of five different Dopamine receptor genes expression (DRD1,DRD2,DRD3,DRD4 and DRD5) in certain diseases of immune and nervous systems have been reported and play multiple role in various disorders such as cancer. Previous controlled studies demonstrated a relationship between changes in dopamine receptor gene expression profiles and clinical improvement. In the present study, it was hypothesized that the dopamine receptor gene expression profile of peripheral blood mononuclear cells in NSCLC patients could be different from those normal individuals which probably increases the risk of NSCLC. Material and methods: We investigated dopamine receptors gene expression changes in peripheral Blood Mononuclear cells (PBMC) of 30 NSCLC patients compared to normal individuals . The PBMC was separated from normal whole blood by Ficoll-hypaque and then the total cellular RNA was extracted and cDNA was synthesized, Real Time PCR was done By using primer pairs specific for five dopamine receptors mRNA and β-actin as internal control . Finally the specificities of the obtaine PCR products for the respective dopamine receptors fragments were comfirmed by sequencing and expression comparing of two group (patients and normal cases) was done by SPSS analysis .Results: the results show that DRD2, DRD3 and DRD4 expression changes profile were significant change in NSCLC patients compared to normal individuals . but DRD1,DRD5 genes didn't show any significant and changes . Conclusions: We conclude that there is a quantitative significant difference of D2-like dopamine receptor genes expression in non small cell lung cancer . On the other hand these significant differences and profiles could contribute in diagnosis, treatment and survey and monitoring of non small cell lung cancer .In addition it could be a lead for design of future therapeutic strategies Key words: Dopamine receptors - Non-small cell lung cancer - Gene expression Citation Format: Mojhgan Sheikhpour, Ghasem Ahangari, Majid Sadeghizadeh, Kian khodadad. Significant changes in D2-like dopamine gene receptors expression associated with non- small -cell lung cancer: A case control study. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5316. doi:10.1158/1538-7445.AM2013-5316