Abstract 5296: FOXP1 expression is associated with tumor progression and poor prognosis in cervical cancer

Abstract

Abstract Background: The forkhead box protein 1 (FOXP1) is considered as both a tumor suppressor candidate and a potential oncogene. Here, we investigated FOXP1 expression in cervical cancer, and the clinical significance of FOXP1 and it's mechanism of action in cervical cancer. Methods: To investigate the potential correlation between FOXP1 and various clinicopathologic parameters, we assessed the expression of FOXP1 in archival tumor tissue specimens from 158 patients with cervical cancer and 280 with cervical intraepithelial neoplasia as well as 378 matched nonadjacent normal tissues by immunohistochemical (IHC) staining. Subsequently, we studied the FOXP1's functional role by small interfering RNA (siRNA) approaches. Results: IHC staining demonstrated that FOXP1 expression increased during the normal to tumor transition of cervical carcinoma (P<0.001), and this increased expression was significantly associated with tumor stage (P = 0.009) and tumor grade (P<0.001). In multivariate analysis, FOXP1+ (P = 0.031) and tumor stage (P = 0.032) were independent prognostic factors for overall survival. FOXP1 knockdown by siRNA decreased cell proliferation, migration, and tumorigenesis in HeLa and CaSki cells. Conclusions: Taken together, our data indicate that FOXP1 has a crucial role in cervical cancer progression, and its overexpression is associated with poor prognosis, supporting that FOXP1 may be used as a promising novel target for therapeutic interventions. Citation Format: Hanbyoul Cho, Woo Kyeom Yang, Sol Kim, Ha-Yeon Shin, Eun Ju Lee, Jae-Hoon Kim. FOXP1 expression is associated with tumor progression and poor prognosis in cervical cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5296. doi:10.1158/1538-7445.AM2015-5296