Abstract 5282: TRPM7, a novel regulator of human nasopharyngeal carcinoma cells Ca2+ influx and migration

Abstract

Abstract Ion channels are involved in various physiologic and pathologic processes, including the migration of tumor cells that is required for metastasis. To determine whether transient receptor potential melastatin 7 (TRPM7) Ca2+ channels play an important role in the migration of tumor cells, we examined the potential function of TRPM7 channels in the migration of 5-8F human nasopharyngeal carcinoma cells. The migratory potential of the cells was impaired by TRPM7 inhibitors (La3+, 2-APB), and their migration was reduced significantly after either TRPM7 was silenced or extracellular Ca2+ was removed. Conversely, addition of TRPM7 activator (BK) promoted the migration of 5-8F cells. Furthermore, the Ca2+ influx regulated by TRPM7 activated release of Ca2+ stores by a calcium-induced calcium release mechanism. This study suggests, first, that Ca2+ influx is required for the migration of human nasopharyngeal carcinoma 5-8F cells. Second, and more importantly, it identifies TRPM7 as a novel potential-regulator of the Ca2+ influx that allows migration of 5-8F cells. TRPM7, therefore, might have potential as a prognostic indicator and as a therapeutic target in cancer. Keywords: TRPM7; nasopharyngeal carcinoma cells; 5-8F; Ca2+ influx; Ca2+; migration. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5282.