Abstract 5273: The cyclin dependent kinase inhibitor p27KIP1 inhibits neuroblastoma cell migration and invasion

Abstract

Abstract Neuroblastoma (NB) is the most common extra-cranial pediatric tumor. Over 70% of NB patients are diagnosed with metastasis, and the survival probability of these patients is significantly lower than patients without metastasis. While the role of p27KIP1 in cell cycle regulation is well documented and conserved in all cell types, there are contradictory reports regarding its function in regulating cell migration and invasion. The purpose of the present study was to elucidate the role of p27KIP1 in regulating migration and invasion of NB cells with MYCN over-expression. Western blot analysis, migration and invasion assays were performed on NB cells with and without over-expression of MYCN. The expression of p27KIP1 was down-regulated using siRNA, and the migration rate was determined on NB cells with MYCN over-expression. Also, the cytoplasmic localization of p27KIP1 in NB cells with and without MYCN over-expression was determined. The results from the current study show that MYCN over-expression increases migration and invasion of NB cells, compared to cells without MYCN over-expression via a process that involves p27KIP1. In addition to its role as a cell cycle regulator, p27KIP1 also regulates NB migration and invasion. This dual role of p27KIP1 as a tumor repressor suggests that it may be an attractive therapeutic target for NB. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5273.