Abstract
Abstract Nucleolus, one of the most important organelles in cell nuclear of eukaryotic cells, is made of rRNA, rDNA and nucleoproteins. Its main function is rRNA synthetic. PES1 is part of nucleolar protein, and was identified in the mutational study of the embryonic development of Zebrafish. Its main biological functions include forming nucleolus, promoting cell proliferation and regulating cell cycle. A few researches indicate the close relationship between PES1 and tumorigenesis. The purpose of this study is to explore the role of PES1 in tumorigenesis and the possible mechanism. Three specific monoclonal antibodies against PES1 were made through hybridoma technique. Using these antibodies, it is found that PES1 had significantly higher expression in gastric and colon carcinoma than in tumor-adjacent and -distant tissues. Immunohistochemical assay on gastric carcinoma specimens and colon carcinoma specimens indicated that PES1 had significantly higher expression in carcinoma than in tumor-adjacent tissues. Moreover, we demonstrated the localization of PES1 was changed from nucleolus to nucleoplasm significantly under the stimulation of chemotherapeutic drugs and UV. The phenomenon of PES1 translocation in nucleus was weaken in drug-resistant cancer cell lines. These results suggest that the translocation of PES1 in nucleus might be related to DNA damage repair and drug resistance mechanism of cancer cells. In summary, PES1 expression is significantly higher in gastric and colon carcinoma tissue. And dynamic changes of PES1 localization in the cell nucleus is an important biological function and is worth further research. Note: This abstract was not presented at the meeting. Citation Format: Qin Feng, Shenyi Lian, Ping Wang, Yue Wang, Zhongwu Li, Chengchao Shou, Tingting Ren, Dongmei Lin. PES1 is highly expressed and its biological function in gastrointestinal tumor [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 5232.
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