Abstract 5182: MSP58 regulates telomerase activity and cell proliferation via interaction with TEIF.

Abstract

Abstract Telomerase expression is essential for cell proliferation and survival, which also plays a role in development, senescence and cancer. In our previous studies, we demonstrated that ectopic expression MSP58 regulates cell proliferation in context-dependent manner and induces premature senescence in HT1080 cells and normal human diploid fibroblasts through p53/p21 senescence pathway. In addition, we also demonstrated that MSP58 overexpression significantly suppressed telomerase activity in HT1080 cells. Currently, we identified a novel MSP58 interacting protein, TEIF (Telomerase transcription element-interacting factor) by yeast-two hybrid screening. TEIF has been reported to up-regulate the hTERT gene expression and telomerase activity. Interestingly, we found that MSP58 represses the TEIF-mediated activation of telomerase. Furthermore, overexpression of MSP58 represses TEIF promoted anchorage-independent growth in HT1080 cells. In contrast, knockdown MSP58 expression increases TEIF promoted anchorage -independent growth in HT1080 cells. These results suggest that MSP58 regulates telomerase activity via interaction with TEIF and contributes to control cell proliferation. Citation Format: Che-Chia Hsu. MSP58 regulates telomerase activity and cell proliferation via interaction with TEIF. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5182. doi:10.1158/1538-7445.AM2013-5182