Abstract 5117: Fluorescence-guided surgery of liver metastasis in orthotopic nude-mouse models

Abstract

Abstract We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were then harvested and implanted to the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Eleven mice with a single liver metastasis were randomized into BLS or FGS groups. Three weeks after the implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Five mice were treated with BLS, six were treated with FGS. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. Citation Format: Takashi Murakami, Yukihiko Hiroshima, Shinji Miwa, Yano Shuya, Makoto Toneri, Mako Yamamoto, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Satoshi Hasegawa, Itaru Endo, Robert M. Hoffman. Fluorescence-guided surgery of liver metastasis in orthotopic nude-mouse models. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5117. doi:10.1158/1538-7445.AM2015-5117