Abstract 5110: Gallic acid induces apoptosis and autophagy in bladder cancer cell

Abstract

Abstract Flavonoid phytochemicals were studied on prevention or therapy in human malignancies including bladder cancer. Here we detected the effect of gallic acid (GA) in human bladder transitional cell carcinoma (TCC, TSGH-8301 cells). In MTT assay, TSGH-8301 cells treated with 30 or 40 μM of GA showed significant decrease of cell viability to about 40 or 20 %. To examine whether the cell death involving apoptosis, we used DAPI stain to observe nuclear condensation and agarose gel electrophoresis to detect DNA fragmentation. GA can cause bladder cell apoptosis. Additionally, the results from flow cytometric analysis showed that GA induced late stage of apoptosis and represented a dose-dependent manner, accompanied by the decrease of Bcl2 and increase of Bax. We also used acridine orange stain and evaluated the expression of LC3, microtubule-associated protein 1 light chain 3, a marker of autophagy, to detect whether GA can induce autophagy in bladder cancer cell. The results showed that GA possesses the ability to induce autophagy in bladder cancer cell in a dose-dependent manner. In conclusion, these results revealed that GA can induce apoptosis and autophagy in human bladder cancer cells. It also implies GA possesses the potential to treat bladder cancer. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5110.