Abstract 5099: Identification of lung cancer biomarkers using lung cancer in female never smokers

Abstract

Abstract On a worldwide level, lung cancer in never smokers (LCINS) occupies 25% of total lung cancer incidence and this disease shows the 7th leading cause of cancer death. It is known that LCINS occurs frequently in asian women. It has been reported that 75% of female lung cancer occurred in never smokers in Republic of Korea. The clinico-pathological and molecular differences as well as geographic and gender variations between lung cancers in never smokers and smokers, indicate that two types of lung cancers should be regarded as a different disease entity. However, data for molecular understanding of LCINS are limited. We designed this study to identify the protein differentially expressed in lung cancer in female never smokers through proteomic analysis including 2-DE and MALDI-TOF-MS. To increase the probability of finding LCINS-associated proteins, the clinical specimens including sera and cancer tissues were classified as the following: normal female smokers, normal female never-smokers, lung cancer in female smokers, and lung cancer in female never smokers. Out of the proteins differentially expressed in LCINS tissues, levels of 10 proteins were increased and those of 8 proteins were decreased. Levels of 6 proteins were increased and those of 5 proteins were decreased in serum of patients with LCINS. The identified proteins were verified by performing Western blot analysis not only in the same sample used in proteomic analysis but also in samples obtained from lung cancer in female smokers. The serum protein, which was difficult to be identified in Western blotting, was verified by ELISA. Seven proteins identified in tissues and serum of patients with LCINS were verified by Western blot analysis and ELISA. These identified proteins might be applicable to biomarker development for diagnosis of LCINS and provide a more comprehensive understanding of tumor maintenance or progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5099. doi:10.1158/1538-7445.AM2011-5099