Abstract 5091: Aneuploidy is in stomatin overexpressed cells

Abstract

Abstract A 31.5 kDa membrane associated protein which is highly abundant in the lipid rafts is stomatin. Human gene expression profile from UCSC Genome Bioinformatics databank indicates that stomatin is major in the peripheral blood, adipose tissue, muscle, and bone marrow. In the beginning, stomatin was thought to be involved in the disease OHSt (overhydrated hereditary stomatocytosis), and therefore named stomatin. However, later studies did not reveal mechanistic role of stomatin in mediating OHSt by evaluating stomatin gene deleted mice; the function of stomatin is therefore remained unclear. In this study, we found that overexpression of stomatin was able to induce the multinucleated cell formation. Our data also demonstrated that stomatin made in the cytoplasm could be transported outside of the cell and released to the culture medium. While adding the culture medium or condition medium to the cells, which did not contain exogenous stomatin, these cells were also triggered to undergo formation of multinucleated cells. Additionally, the stomatin induced multinucleated cells presented aneuploidy and abnormal centrosome number. Possible mechanisms underlying functions of stomatin that induce multinucleated cells are investigated. Note: This abstract was not presented at the meeting. Citation Format: Jui-Hao Lee, Chi-Hung Lin. Aneuploidy is in stomatin overexpressed cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5091. doi:10.1158/1538-7445.AM2014-5091