Abstract 5076: Multi-protein tissue and blood plasma protein marker patterns of endometrioid endometrial and squamous cervical cancers

Abstract

Abstract Endometrial cancer is the second spread cancer of women in Western Europe and Nothern America. There is no screening developed for early detection of endometrial cancer, its major types and precursor lesions. We used proteomics-based methods including two-dimensional gel electrophoresis of tissue and blood plasma proteins, analyses of gels by SameSpot (Nonlinear) software and MALDI-TOF MS-assisted identification of proteins. Measurement of DNA ploidy was used for the selection of genomic subtypes of endometrial cancer. Expression of identified proteins was evaluated in endometrial cancers, atypical and simple hyperplasia and normal endometrium using immunohistochemistry. Tumor tissue biopsies of endometrial cancer, normal endometrium, squamous cervical cancer, and squamous cervical epithelium as well as low-abundant blood plasma proteins of the same patients were used for two-dimensional gel electrophoresis. We also analyzed published data on chromosomal changes in early and prognostically unfavorable endometrial cancer and corresponded proteins identified by us to the corresponding coding genes. One hundred and thirty differentially expressed tissue proteins were identified as being related to malignant transformation towards endometrial and cervical cancers. Among them is a group of proteins that specifically differentiate genomically stable and unstable endometrial cancers. Many similarities between the protein expression were found for genomically unstable endometrial cancer and squamous cervical cancer which in most cases are genomically unstable. Moreover, inclusion of squamous cervical cancer cases made it possible to select proteins that are specific for endometrial cancer and proteins which characterize non-malignant tissues. Using available data on chromosomal changes in endometrial cancer we have selected proteins that are markers of prognostically unfavourable endometrial cancer and that change their expression already in atypical hyperplasia of the endometrium. By analysis of low-abundant blood plasma proteins we have identified 18 marker proteins that differentiate endometrial cancer from cervical cancer. In conclusion, proteins differentially expressed in tissue of endometrial cancer include patterns that are typical for genomically stable and unstable subtypes and changes in their expression are detectable already in atypical hyperplasia of the endometrium. Endometrial and cervical cancers are characterized by differential blood plasma protein expression patterns. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5076. doi:10.1158/1538-7445.AM2011-5076