Abstract
Abstract MicroRNA (miRNA) expression profiling and quantitative real-time PCR (qRT-PCR) from fine needle aspirates (FNA) of formalin-fixed paraffin-embedded cell blocks (FFPE) could be a major improvement for prognosis and predicting treatment response in inoperable tumors of pancreatic cancer (PC). MicroRNA (miRNA) profiling provides a more comprehensive molecular interrogation of tumors. In the present study, miRNA expression profiling was performed on formalin-fixed paraffin-embedded cell blocks (FFPE) from FNA of suspicious pancreatic masses. Subjects were retrospectively studied and included those who had a pathological diagnosis of pancreatic adenocarcinoma and others with no histological proof of any cancer. We compared the expression profile of miRNAs in the pooled samples of FNA blocks from patients diagnosed with PC (n=29) with normal controls (n=15). MicroRNA profiling confirmed the deregulation of over 228 miRNAs of which, the top seven were validated by qRT-PCR. The expression of let-7c, let-7f, and miR-200c were significantly lower in almost all of the 29 patients compared to normal samples. On the contrary, the expression level of miR-486-5p, and miR-451 were significantly higher in all PC samples. Interestingly, two of the miRNAs, let-7d and miR-423-5p, that showed significant down-regulation or up-regulation in pooled sample showed a differential expression between the samples with individual qRT-PCR. These results suggest that identifying seven-miRNA signatures for PC in FNA cell blocks and validating with qRT-PCR can provide a strategy for improving the diagnosis of PC by using minimally invasive procedures. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5027. doi:1538-7445.AM2012-5027
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
