Abstract 4953: A proposal to extend standardized organ mapping antibody panels (OMAPs) to integrate protein and RNA analysis in spatial biology

Abstract

Abstract The HubMap consortium has developed a new standard to report on normal histological samples using multicolor immunofluorescence imaging. An Organ Mapping Antibody Panel (OMAP) currently describes in a table the antibodies used, the cycle number assigned to a given reagent, and provides the rationale for using a particular antibody to better understand features of the tissue. This table is accompanied by antibody validation templates that show example images from the tissue described for the OMAP table. In addition, links to existing databases, gene symbols, the antibody features and colors are included. For each tissue, a further table called "Anatomical Structures, Cell Types, plus Biomarkers" (ASCT+B), which contains structures of the tissue, cell types in a given tissue structure, key RNA transcripts for a given cell type, and antibody stains as used in the OMAP table, is created. So far, this process has not been established for spatial multiomic datasets including protein and RNA detected on the same tissue section. We have extended spatial biology methodology to combine cyclic RNA transcript detection with cyclic antibody-based protein detection using the MACSima Imaging Cyclic Staining (MICS) technology as well as H&E-staining on the same tissue section. For such datasets, the current OMAP data table needs to be extended by adding information on the RNA detection, cycle IDs and RNA validation templates, demonstrating the correct performance of a given RNA probe detection. This poster will demonstrate a proposal to extend the OMAP table structure on an extended version of the “tonsil OMAP (OMAP-10)”, which was published on the Zenodo data repository page (https://zenodo.org/records/7875938). In addition, we will apply this extended OMAP to a colorectal cancer sample, to highlight the applicability and relevance of the OMAP structure for tumor tissue. Citation Format: Werner Müller, Julia Femel, Emily Neil, Dongju Park, Rebecca C. Hennessey, Eric C. DiBiasio, Michael DiBuono, Hanna Lafayette, Erica Lloyd, Hsinyi Lo, Alex Makrigiorgos, Sameh Soliman, Dominic Mangiardi, Paurush Praveen, Silvia Rüberg, Fabian Staubach, Ryan Hindman, Thomas Rothmann, Hansueli Meyer, Tanya Wantenaar, Jinling Wang, Robert Pinard, Andreas Bosio. A proposal to extend standardized organ mapping antibody panels (OMAPs) to integrate protein and RNA analysis in spatial biology [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4953.