Abstract 4932: Exploration of EMT-related microRNAs using function-based screening with expression analysis of E-cadherin in Panc1 line

Abstract

Abstract Recently, epithelial-mesenchymal transition (EMT) has been demonstrated to contribute to malignant transformation during cancer progression. To perform the function-based screening of EMT-related microRNAs (miRNAs), we first generated an in vitro model using a pancreatic cancer cell line, Panc1, in which a transcriptional reporter construct containing E-cadherin promoter region and a reporter gene, GFP, was transfected. After the selection of independent clones by the limiting dilution and the examination of GFP-upregulation in response to overexpression of ectopic miR-200b/a, we successfully established a stable transfectant clone, Panc1-EPG8. Then, we performed function-based screening with expression analysis of GFP in Panc1-EPG8 cells 4 days after ectopic transfection of 470 synthetic miRNAs. We further reexamined the expression level of E-cadherin after ectopic transfection with selected candidate miRNAs in Panc1-EPG8 cells by Western blotting and qRT-PCR, resulting in the identification of a novel miRNA as a candidate EMT-related miRNA. Our findings strongly suggest that this miRNA may play a crucial role during EMT in cancer progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4932. doi:1538-7445.AM2012-4932