Abstract 4906: Tamoxifen-mediated apoptosis relationship with ERα36 in gastric cancer cell BGC823

Abstract

Abstract Aim: The growth, apoptosis and estrogen receptor α36 (ERα36) expression and location changes in gastric cancer cell line BGC823 were observed, when dealt with different concentrations Tamoxifen (TAM), to characterize the role of TAM in gastric cancer growth and apoptosis and the mechanism of it. Materials and Methods: BGC823 cell was dealt with 10-6M and 10-5M TAM for 24h and 48h. Cell growth was assessed by WST1 assay. The mRNA and protein expressions of ERα36 were examined by RT-PCR and Western blot methods. Average optical densities were detected and processed using Student's t test analysis. Immunofluorescence assay was used to examine ER-α36 location in BGC823 cell. Hoechest33258 assay was used to examine apoptosis. Results: TAM could promote apoptosis and inhibit growth of BGC823 cell, and this effect had concentration dependant (P<0.05). TAM could promote ERα36 mRNA expression, and the increase level of ERα36 mRNA expression had concentration dependent of E2β(P<0.05). TAM could promote ERα36 protein expression, and the increase level of ERα36 protein expression had concentration dependent of E2β(P<0.05). The location of ERα36 had no change, when BGC823 cell dealt with TAM.Conclusions: TAM could promote apoptosis and inhibit growth of BGC823 cell, and the effect had concentration dependant. TAM could promote ERα36 expression in BGC823 cell, and the effect had concentration dependant. ERα36 may participate apoptosis mediated by Tamoxifen in Gastric Cancer Cell Line BGC823 Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4906. doi:1538-7445.AM2012-4906