Abstract
Abstract Background: Prostate cancer progression is a complex process in which chemokines and their receptors play an important role related to tumor survival, growth, and metastasis. Objectives: 1) To determine the expression profiles of proinflammatory chemokines in prostate cancer cell line supernatants and their association with the metastatic phenotype, and 2) To determine whether these chemokines are present in the exosomes released from prostate cancer cell lines examined. Methods: Prostate cancer lines investigated included PWR-1E (non-tumorigenic), LNCaP (low metastatic potential) and PC-3 (high metastatic potential). The Human Common Chemokines Multi-Analyte ELISArray Kit (Qiagen) was used to evaluate the expression profiles of several proinflammatory chemokines in the supernatant and in the exosomal fraction of prostate cancer cells in culture. Chemokine profiles were correlated with the metastatic abilities of the cell lines analyzed. Chemokines evaluated were CCL2, CCL3, CCL4, CCL5, CCL11, CCL17, CCL22, CXCL1, CXCL8, CXCL9, CXCL10 and CXCL11. Results: Differential chemokine profiles in the cell lines were observed in the supernatant and in the exosomal fractions. Conclusions: Prostate cancer cell lines with different phenotypes have different chemokine profiles, both in supernatant and in the exosomal fraction of these cells in culture. These results may be useful for the design of diagnostic, prognostic and/ or therapeutic strategies. Financial support: Universidad de Cartagena Project Code: Res 04670 - 2012 Citation Format: Niradiz Reyes, Oscar Correa, Alfonso Bettin, Raj K. Tiwari, Jan Geliebter. Expression profiles of proinflammatory chemokines in prostate cancer cell supernatants. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4882. doi:10.1158/1538-7445.AM2014-4882
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