Abstract 4863: Streamlining NGS workflows using cancer samples by the application of the DNA Integrity Number (DIN) from the Genomic DNA ScreenTape assay

Abstract

Abstract The results of numerous molecular screening and assay methods often rely on the overall quality of the genomic DNA (gDNA) input material. However extraction of genetic material can be challenging and often results in low amounts or variable quality of gDNA samples, which are further subjected to time and cost intensive downstream applications. For example, array comparative genome hybridization (aCGH) and Next Generation Sequencing (NGS) can require intact, high quality gDNA to ensure high quality, unambiguous results. It is therefore widely recommended to perform an initial quality control (QC) of the input material. Especially as only the final step of these workflows reveals if meaningful results have been achieved. In order to provide an objective and automated measure to standardize the gDNA integrity assessment, a software algorithm has been developed. This functionality of the 2200 TapeStation system provides a numerical determination of the gDNA integrity and is referred to as the DNA Integrity Number (DIN). This study demonstrates how DIN obtained by the upfront QC of gDNA on the Agilent Genomic DNA ScreenTape assay has allowed for significant saving of sequencing and sample preparation overhead using cancer samples in NGS workflows. Citation Format: Eva Schmidt, Isabell Pechtl, Barry McHoull, Melissa Liu. Streamlining NGS workflows using cancer samples by the application of the DNA Integrity Number (DIN) from the Genomic DNA ScreenTape assay. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4863. doi:10.1158/1538-7445.AM2015-4863