Abstract 4849: PPARγ and FoxO1 expression and activity in aqueous cinnamon extract treated cells

Abstract

Abstract Peroxisome-proliferator activated receptor (PPARγ) has been proven to have inhibitory effects on tumor angiogenesis. Research has demonstrated that natural PPARγ ligands are anti-tumorigenic due to growth inhibition and anti-proliferative effects. Cinnamon is one potential PPARγ ligand tested to explore its effects on PPARγ and FoxO1 expression and activity. Literature also suggests that breast cancer proliferation is increased with obesity and co-cultures of adipocytes with MCF-7 cells have suggested an increase in lipase activity. We therefore sought to explore the effects of cinnamon on these transcription factors and lipase activity in 3T3-L1 cells to determine if cinnamon is a plausible co-therapeutic for breast cancer patients. 3T3-L1 cells were cinnamon treated for 48-96 hours post differentiation. Nuclear protein was extracted for ELISA quantification of PPARγ and FoxO1 binding potential. Cytosolic and nuclear protein extracts were analyzed via Western blot of FoxO1 and PPARγ. Copy DNA was produced from the total RNA and expression levels of PPARγ and FoxO1 were examined by TaqMan real-time PCR assays. A lipase activity assay was performed on freshly extracted protein from cultured cells. Western blot data demonstrates an increase in FoxO1 protein with cinnamon treatment at 96 hours post differentiation. PPARγ data shows no significant changes in protein quantity, however ELISA data suggests increases in binding potential PPARγ as well as FoxO1 transcription factors with cinnamon treatment. Preliminary expression analysis suggests changes in both PPARγ and FoxO1. Lipase activity data suggests a significant increase in cinnamon treated adipocytes. Data from the study suggests that cinnamon activates PPARγ and that it may play a role in decreasing tumorigenic activity. However, with particular concern in breast cancer cells, the activation of PPARγ in adipocytes appears to increase the lipase activity, thus producing more free fatty acids and potentially enhancing energy dependent proliferation. Citation Format: Sean Mild, Cole Swartz, Amy Aulthouse, Amy Stockert. PPARγ and FoxO1 expression and activity in aqueous cinnamon extract treated cells. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4849.