Abstract 4785: Deficiency of cystathionine beta synthase gene does not affect tumor formation and survival of Eμ-myc mice

Abstract

Abstract Aberrant DNA methylation is a hallmark of many types of cancers. S-adenosylhomocysteine (AdoHcy) is a strong inhibitor of DNA methytransferases. Deficiency of cystathionine beta synthase gene (Cbs) causes elevation of AdoHcy in variety of tissues. Here we have generated an Eμ-myc mouse model which is either wild type, heterozygous or homozygous for a deletion allele of Cbs (Eμ myc+ Cbs+/+; Eμ myc+ Cbs+/−; Eμ myc+ Cbs−/−). Homozygous Cbs deficient mice in both Eμ myc+ and Eμ myc− background were born at Mendelian frequency indicating that there is no synthetic lethality between Cbs and Eμ myc. In aging, we observed no significant difference in survival of Eμ myc+ Cbs+/+, Eμ myc+ Cbs+/− and Eμ myc+ Cbs−/− mice (Median survival of 150, 149 and 117 days respectively). However, preliminary data shows that tumor tissue from Eμ myc+ Cbs−/− animals has greatly elevated levels of AdoHcy compared to other genotypes. Our studies suggest that elevated levels of AdoHcy has no effect on tumor formation in Eμ myc mice. Citation Format: Sapna Gupta, Warren D. Kruger. Deficiency of cystathionine beta synthase gene does not affect tumor formation and survival of Eμ-myc mice. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4785. doi:10.1158/1538-7445.AM2015-4785