Abstract 4752: Induction of cytotoxic T lymphocytes primed with tumor RNA-loaded dendritic cells in esophageal squamous cell carcinoma: Preliminary step for DC vaccine design

Abstract

Abstract Backgrounds: Dendritic Cells (DC) are potent antigen presenting cells with the ability to prime naïve T cells and convert them to cytotoxic CTL. We evaluated the capability of autologous DCs transfected with total Tumor and normal RNA to induce cytotoxic CTL as the preliminary step to design DC-based vaccine in the esophageal squamous cell carcinoma (ESCC). Methods: Monocytes-derived DCs were electroporated with either total tumor RNA or normal RNA. T cells were then primed with tumor RNA transfected DCs and lysing effects of the generated CTL were measured with Cytotoxicity assay and IFN-γ Release Elispot Assay. Results: Cytotoxicity against DCs loaded with tumoral RNA was induced (%24.8±5.2 SEM) while in normal RNA-loaded DCs it was minimal (%6.1±2.4 SEM) and significantly lower (p < 0.05). INF-γ secretion was more than 2-folds higher in tumoral RNA-loaded DCs when compared with normal RNA-loaded DCs (p < 0.05). Conclusion: Electroporating DCs with tumor RNA made tumor antigen presenting cells that enhanced cytotoxicity effects of T cells against ESCC. This may be a useful autologous ex vivo screening tool for confirming the lytic effects of primed T cells on tumor and evaluate probable further adverse effects on noncancerous tissue. These data are a crucial preliminary step to establish a total tumor RNA-pulsed DC vaccine therapy of ESCC. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4752.