Abstract 472: Positive Role of Combined Exercise Training in a Model of Metabolic Syndrome and Menopause: Autonomic, Inflammatory and Oxidative Stress Evaluations

Abstract

The aim of this study was to evaluate the effects of combined exercise training in hypertensive ovariectomized rats submitted to fructose overload. Wistar and spontaneously hypertensive (SHR) rats were divided into (n=8/group): control (C), hypertensive (H), hypertensive ovariectomized (HO), hypertensive ovariectomized submitted to fructose overload sedentary (HOF) and trained (HOFT). The combined exercise training was performed on a treadmill and ladder adapted to rats in alternated days, during 8 weeks. At the end of exercise protocol, arterial pressure (AP) signals were directly recorded. Cardiovascular autonomic modulation was evaluated by spectral analysis. Oxidative stress and inflammation were measured on cardiac tissue. The association of hypertension and ovariecyomy induced an increase in arterial pressure (AP) (HO: 164±5 vs. H: 146±6 mmHg) and body weight in relation to hypertensive rats. The association of risk factors (hypertension+ovariectomy+fructose) induced an additional increase in AP (HOF: 174±4 mmHg), increased heart rate (HOF: 403±12 vs. HOS: 348±16 bpm) and blood triglycerides (HOF: 160±8 vs. HO: 125±6.4 mg/dl), promoted impairment on the insulin sensitivity, cardiovascular autonomic modulation, oxidative stress (redox ratio of glutathione: HOF: 8.94±0.8 vs. HO: 13.00±1.4) and inflammation (TNF-α- HFO: 65.8±9.9 vs. HO: 31.7±8.6 pg/mg protein) of these animals. However, the combined exercise training was able to reduce the AP (HOFTc: 158±4 mmHg) and heart rate (HOFT: 303±5 bp), to normalize the triglyceride levels (HOFT: 137±4.7 mg/dL), insulin sensitivity and vascular autonomic modulation. Moreover, this approach reduced cardiac lipoperoxidation and increased antioxidant defense and redox balance of glutathione (HOFT: 11.94 ± 1.1), reduced TNF-α (HOFT: 33.1±4.9 mg/dL) and increased IL-10. The results of this study demonstrated that the association of risk factors promoted additional impairment in metabolic, cardiovascular, autonomic, inflammatory and oxidative stress parameters and the combined exercise training was able to reduce and/or normalize these dysfunctions.