Abstract 4575: Açaí intake reduces the aberrant crypt foci development in a colitis-associated carcinogenesis model in male Wistar rats

Abstract

Abstract Colorectal cancer is a main cause of cancer death in the western world. In Brazil, it is the third more common neoplasia in both men and women (INCA, 2014). In our previous study (Fragoso et al., Food Chem Toxicol., 2013), we showed that spray-dried açaí (Euterpe oleraceae Mart.) pulp intake retarded the development of colon cancer in male Wistar rats. Therefore, the objective of this research was to evaluate the potential protection of dietary freeze-dried açaí pulp using a chemically-induced colon carcinogenesis and colitis-associated model in male Wistar rats. The animals were randomly distributed into four groups: G1, DMH [dimethylhydrazine] + TNBS [2,4,6-trinitrobenzene sulfonic acid] + basal diet; G2, DMH + TNBS + basal diet with 5% açai pulp; G3, DMH + TNBS + basal diet with 7.5% açai pulp; G4, DMH + TNBS + basal diet with 0.2% of NAC [N-acetylcysteine]. The acute colitis was induced with the administration of 10 mg of TNBS dissolved in 0.25 ml 50% ethanol (v/v) by means of a Teflon cannula inserted 8 cm into the anus. At the end of the 25th week, animals were sacrificed for evaluation of Aberrant Crypt Foci (ACF) in the colon. Unsenctioned and fixed colons were analyzed for total aberrant crypt (AC) and total ACF and ACF multiplicity under methylene blue staining. The results were analyzed by ANOVA followed by post hoc Tukey test. A significant reduction in total ACF and AC for G2 when compared to G1 (p ≤0.001) was seen. Regarding the ACF multiplicity, it was observed a significant reduction in ACF with 4-10 aberrant crypts for G2 and G4 and for ACF with >10 aberrant crypts for G2-G4 groups in relation to the G1 group (p = ≤0.001, respectively). Also, it was observed a significant reduction in the number of total AC in the distal colon from the G2 and G4 groups compared to G1 group (p = 0.003) and in the medial colon from the G2 group compared to G1 group (p = 0.016). These results suggest that açaí intake may reduce the number of AC and ACF and the number of ACF with more than four aberrant crypts in this chemically-induced colon carcinogenesis and colitis-associated model in rats. Citation Format: MARIANA F. FRAGOSO, GUILHERME R. ROMUALDO, CLAUDIA H. PELLIZZON, LUIS F. BARBISAN. Açaí intake reduces the aberrant crypt foci development in a colitis-associated carcinogenesis model in male Wistar rats. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4575. doi:10.1158/1538-7445.AM2015-4575