Abstract 4425: Analysis of host-Epstein-Barr virus microRNA expression in nasopharyngeal carcinoma

Abstract

Abstract In the tumorigenesis process, miRNAs could act as oncogenes or tumor suppressor genes in the cancer cells by regulating miRNA target genes. Nasopharyngeal carcinoma (NPC) associated Epstein-Barr virus (EBV) can also influence the occurrence of NPC through its miRNAs. In this study, we used the next generation sequencing technology to analysis host-viral miRNA profiles in 24 NPC and 5 adjacent normal tissues. In average, 10.62% of mappable reads mapped to EBV genome in NPC tissue. Most of the small RNAs in NPC tissues which mapped to EBV genome were miRNAs (95.51%), rest of them were EBERs (1.80%) and v-snoRNA1 (2.68%). The percentages of EBV miRNA in total human and EBV mature miRNA reads were ranged from 4% to 51% (average 29.61%). For EBV miRNAs, the expression level of BART10-3p and BART9-3p were the top two in NPC tumors. BART20-5p, BART19-3p and BART21-5p were correlated to tumor stage. On the other hand, there were 11 and 34 human miRNAs differentially up- and down-regulated in NPC tissue, respectively. The present research provides the first evidence obtained through direct sequencing to systematically and comprehensively describe the expression of EBV and human miRNAs in NPC tissue. Citation Format: Wen-Hui Su, Jia-Hao Zhang, Kai-Ping Chang. Analysis of host-Epstein-Barr virus microRNA expression in nasopharyngeal carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4425. doi:10.1158/1538-7445.AM2017-4425