Abstract 442: Strenuous Exercise And Polyphenol Intake Differentially Modulate Lipoproteins

Abstract

Objective: To investigate gene expression levels of ATP-binding cassette transporter A1 (ABCA1), proprotein convertase subtilisin/kexin type 9 (PCSK9), paraoxonase 1 (PON1), angiopoietin-like 3 (ANGPTL3), angiopoietin-like 4 (ANGPTL4), peroxisome proliferator-activated receptor gamma (PPARγ), apolipoprotein C-III (APO CIII), and apolipoprotein A-5 (APO A5) after 30 days of quercetin intake combined with exercise. Methods: Forty C57BL/6 mice were divided into four groups (n=10): no exercise and no quercetin (C); quercetin and no exercise (Q); exercise and no quercetin (E); and exercise combined with quercetin (EQ). All animals were fed a high-fat diet containing 1.5% cholesterol as part of a 42% fat diet without antioxidants. After 2 months the animals were sacrificed and liver tissues were analyzed for ABCA1, PCSK9, PON1, ANGPTL3, ANGPTL4, PPARγ, APO CIII, and APO A5 expression using qPCR and western blot. Results: After the treatment period ABCA1, PPARγ, APO A5, and ANGPTL4 mRNA expressions differentially altered with exercise and quercetin intake. The results also indicated that PCSK9 mRNA expression was up regulated in the combination group; however, this did not correlate with an increase in secreted protein. Finally, the level of PON1 protein expression was reduced in the exercise quercetin combination group compared to the control. Conclusions: Hepatocyte lipoprotein expression levels were influenced by quercetin consumption combined with exercise. The changes were not consistent with the perceived effects of the exercise polyphenols combination.