Abstract
Abstract CD150 is a member of the SLAM family that recently has been reported to be differentially expressed among primitive progenitors in the bone marrow of adult mouse. It was utilized to purify hematopoietic stem cells using a simple combination of SLAM family members. To elucidate the presence of this family on acute lymphoblastic leukemia (ALL), we analyzed the expression of this family member on human B-ALL progenitor cells, as well as its relationship with the engraftment of leukemic blasts in serial xenotransplantation assay. Expression analysis was carried out by flow cytometry. We combined the expression pattern of human CD150, CD244 and CD48 with serial xenotransplantation of B-ALL progenitor cells to indicate their relationship. Our data showed that CD48 and CD244 were expressed on most B-ALL progenitor cells, and interestingly only blast cells with low percentage of CD150+ population were able to reconstitute leukemia into primary, secondary and tertiary NOD/SCID mice. In conclusion, SLAM family members are present on B-ALL progenitor cells and the engraftment potential of leukemic blasts is correlated negatively with the proportion of CD150+ cells, the percentage of which can serve as a useful predictor for graft success of B-ALL to immune deficient mice. The mechanism of CD150 as a predictor is under further investigation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4299. doi:10.1158/1538-7445.AM2011-4299
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