Abstract 4281: Real-time imaging of the cell-cycle in vivo

Abstract

Abstract FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) cells are labeled orange in the G1 phase nuclei (monomeric Kusabira-Orange2: mKO2) and labeled green in the S/G2/M phases (monomeric Azami-Green1: mAG1) (Cell 132, 487-498, 2008). FUCCI-expressing MKN45 gastric cancer cells were used for imaging cell-cycle dynamics in vivo and in vitro. When a confluent monolayer of MKN45-FUCCI was scratched, the edge of the wound turned green within 24hr, indicating that healing of the wound required proliferation of MKN45 cells. FUCCI-expressing MKN45 cells were injected subcutaneously in the nude mice. The injected cells gradually grew and emitted both green and red fluorescence for 14 days, indicating cell cycle progression. In the chorioallantonic membrane (CAM) chick embryo model, green and red fluorescence FUCCI cells and blood vessels were observed for 5 days. FUCCI cells of the edge in the tumor migrated into the transparent egg membrane. Mouse and CAM FUCCI tumor models will be used to study the role of cell cycle dynamics in metastasis including dormancy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4281. doi:10.1158/1538-7445.AM2011-4281