Abstract
Abstract Human cell lines are widely used in biological and biomedical research, and accurate data interpretation depends on unambiguous identity of a cell line relative to its original source. Contamination and misidentification of human cell cultures has been identified as a long-standing issue within the scientific community, and steps are now being taken to address the problem. Short-tandem-repeat (STR) DNA profiling provides an accurate, reliable, and standardized method for authentication of human identity. Currently, the ATCC is considering proposed standards for cell line authentication utilizing human STR typing, and many journals (including all AACR published journals) have adopted the NIH-recommended policy to mandate cell line authentication as a requirement for publication. Here we demonstrate the validation and use of two different STR systems to authenticate human cell lines as well as detect contamination by mouse feeder cells and other human cell lines. Threshold levels for detection of complete profiles as well as contamination are compared between the systems, and typical typing issues specific to cell lines are discussed. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4256. doi:1538-7445.AM2012-4256
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