Abstract 4187: Expression of ion channels in a cervical cancer mouse model

Abstract

Abstract Introduction. Because some ion channels have been suggested as early tumor markers and therapeutic targets, in the present study we examined the expression of ion channels in the cervical cancer transgenic mouse model K14E7 expressing the HPV16 E7 oncogene under the control of the human keratin 14 promoter. Methods. We used the K14E7 transgenic mouse; some groups were treated with 17β-estradiol. Continuous release pellets delivering 0.05 mg of 17β-estradiol were implanted into 4-6 weeks old female mice during 90 days to obtain low grade cervical dysplasia or during 180 days to produce cervical cancer. The cervix was removed at the end of the treatment. Protein and mRNA expression of 4 ion channels (Cav3.1, Cav3.2, Kv10.1, KCa1.1) were analyzed by qRT-PCR and immunohistochemistry, respectively. Results. We found increased expression of Cav3.1, Kv10.1 and KCa1.1 in mice with cervical dysplasia in comparison to control animals. This increase was even higher in animals with cervical cancer. In contrast, Cav3.2 expression decreased in animals with cervical dysplasia and increased in mice with cervical cancer. We also found overexpression of the KCa1.1 protein in the transformed zone of the cervical cancer group, but not in the control group. Conclusions. The channel expression profile here studied might serve as a cervical cancer marker. This work was partially supported by Conacyt grant 141126 to JC. Citation Format: ANA RAMIREZ, EUNICE VERA, PAUL LAMBERT, PATRICIO GARIGLIO, JAVIER CAMACHO. Expression of ion channels in a cervical cancer mouse model. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4187. doi:10.1158/1538-7445.AM2015-4187