Abstract
Structural and biochemical changes in stored platelets are influenced by collection and processing methods. During platelet concentrate storage, lesions can appear of which certain can be involved in adverse reaction after transfusion. The aim of this study was to investigate the lipidome in the supernatant of platelet concentrates (PCs) dependent on processing and storage conditions comparing apheresis (SDA) versus buffy coat (BC) platelet concentrate (PC) methods. We investigated various lipid mediator accumulation (Lysophospholipids and Eicosanoids), in either platelet concentrates (SDA-PCs and BC-PCs) stored from 0 to 5 days. All components were produced following French Blood Establishment protocols according to European (EDQM-GTS) Standards; lipids were measured with LS/MS spectrometry. Our data showed no difference between SDA-PC and BC-PC regarding lysophosphatidic acid; however, lysophosphatidylcholine levels were higher in BC-PC than in SDA-PC. Other eicosanoids were affected by either collection or storage conditions of PC. Along the storage, some lipid mediator levels are modulated in SDA-PC and BC-PC. It could be defined as standard platelet components ready for transfusion. To what extent differences, even minimal, matter in clinical outcome remains to be evaluated.
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