Abstract 4103: Ptprz gene encoding a transmembrane-type protein phosphatase is up-regulated by Sox-2 in glioma.

Abstract

Abstract Ptprz is a receptor-like protein-tyrosine phosphatase (PTP) abundantly expressed in neuronal and glial cells in the central nervous system. To date, several potential substrates of Ptprz such as p190RhoGAP, ErbB4, and Git-1, have been identified. In terms of its biological functions, for example, it was shown that Ptprz regulates differentiation of glial cells, but its role(s) in gliomagenesis remains obscue. In this study, expression of Ptprz mRNA in human glioma samples were analyzed. Ptprz mRNA was up-regulated in glioma when compared to normal brain. We found that the levels of Ptprz mRNA in specimens are correlated with those of Sox-2, a transcription factor associating with glioma stemness. Knock-down of Sox-2 by siRNA decreased Ptprz mRNA of several glioma cell-lines. ChIP analysis showed the physical association of Sox2 to the putative promoter region of Ptprz gene, suggesting that Ptprz is a direct transcriptional target of Sox-2. Up-regulation of Ptprz expression may be a part of transcriptional program elicited by Sox-2, which may contribute to glioma stemness Citation Format: Yoji Yamashita, Nobuhiro Tanuma, Toshihiro Kumabe, Teiji Tominaga, Hiroshi Shima, Ryuichi Katakura. Ptprz gene encoding a transmembrane-type protein phosphatase is up-regulated by Sox-2 in glioma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4103. doi:10.1158/1538-7445.AM2013-4103