Abstract 4062: Human peptide deformylase expression and activity in various cancers

Abstract

Abstract Human peptide deformylase (hsPDF) expression has been detected in human cells, but it is unclear if the enzyme is functional. Our hypothesis is that hsPDF is functional in human cells. The objectives of this study were to 1) quantify hsPDF transcript levels in normal and cancerous cells and tissues and 2) determine the effects of hsPDF inhibition on the proliferation of normal and cancerous cells. Quantitative real-time PCR was used to measure hsPDF mRNA levels in normal and cancerous cell lines and tissues. Increases in hsPDF mRNA expression were seen in most cancer cell lines compared to the normal. hsPDF was up-regulated in most, but not all, cancer tissues compared to the normal, with the greatest differences observed in breast, colon, and lung tissues. Various cancer and normal cell lines were treated with increasing concentrations of a known PDF inhibitor, actinonin, and cell proliferation was assessed. The antiproliferative effects of actinonin were greater in prostate, colon, and breast cancer cell lines than in the normal cell lines. These results suggest that hsPDF is up-regulated in certain cancers, and that it may play an active role in the proliferation of human cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4062.