Abstract 4027: Traditional Chinese Medicine herbal mixture LQ prevents cyclophosphamide-induced metastasis and toxicity

Abstract

Abstract Cyclophosphamide (CTX) can enhance metastasis (Cancer Res. 66, 303-306, 2006; Cancer Res. 68, 516-520, 2008) and has toxic side effects such as immune-suppression, weight loss, and hair loss. In the present study, the traditional Chinese medicine (TCM) herbal mixture LQ (J. Cell. Biochem. 114, 2131-2137, 2013) was tested to determine if it could enhance CTX efficacy and reduce side effects. HT1080 human fibrosarcoma cells, labeled with GFP in the nucleus and RFP in the cytoplasm, were injected into the epigastric cranialis vein of nude mice that were pre-treated with CTX and LQ. Lewis lung carcinoma (LLC)-RFP cells were injected into the tail vein of mice that were pre-treated with CTX and LQ. Efficacy was monitored with dynamic subcellular imaging of trafficking of cancer cells in blood vessels (Cancer Res. 68, 516-520, 2008) and imaging metastasis to the lung. LQ significantly reduced CTX-induced lung metastasis and cancer-cell adhesion to the inner wall of blood vessels. LQ also reduced kidney toxicity induced by CTX. Treatment with LQ induced hair growth after CTX administration. This result demonstrates that LQ provides safe and effective prevention of metastasis and toxicity in combination with chemotherapeutic agents such as CTX. Citation Format: Lei Zhang, Chengyu Wu, Lingna Li, Yong Zhang, Robert M. Hoffman. Traditional Chinese Medicine herbal mixture LQ prevents cyclophosphamide-induced metastasis and toxicity. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4027. doi:10.1158/1538-7445.AM2014-4027