Abstract 3979: Active components of Alpinia katsumadai-induced cell apoptosis and autophagy in human pancreatic cancer cells

Abstract

Abstract Alpinia katsumadai, as a traditional Chinese medicine, exhibited potent anti-oxidative and anticancer effects. Studies have shown that the effect of the active components of Alpinia katsumadai (ACAK) on anticancer is via apoptosis or autophagy in colorectal and glioblastoma cancer cells. However, the effects of ACAK on anticancer effect and inducation of apoptosis and autophagy in pancreatic cancer cells are still unknown. Therefore, we investigated the effects of ACAK on cytotocixity, apoptosis and autophagy as well as associated mechanisms in pancreatic cancers Panc-1 and Panc-28 cells. We found that ACAK showed greater inhibitory effects on the proliferation of Panc-1 and Panc-28 cells in a dose- and time-dependent manners (IC50 0.1692 and 0.1554mg/ml, respectively) compared to a variety of other cancer cells including breast cancer MDA-MB-468 cells, melamoma A875 cells, lung cancer A549 cells, colon cancer HCT-116 cells, and glioblastoma cancer U87 cells (IC50 0.2302, 0.2586, 0.2642, 0.3006, and 0.4298, respectively) by CCK-8 assay. Furthermore, ACAK induced cell apoptosis in pancreatic cancer Panc-1 and Panc-28 cells in a dose- dependent manner by Hochest 33342-PI and AnnexinV-PI dual staining analysis. Moreover, the expression levels of apoptosis related proteins casepase 3, casepase 9, and PARP were significantly up-regulated by Western blotting analysis. The study of cell autophagy was dectected by mRFP -GFP-LC3 adenovirus and western-blotting. and the results showed that the expression levels of autophagy-related protein beclin-1 and LC3-II were significanlt up-regulated by mRFP-GFP-LC3 adenovirus and western-blotting analysis. Our findings demonstrated that ACAK had greater cytotoxicity than a variety of other cancer cells by inducing apoptosis and autophagy in pancreatic cancer Panc-1 and Panc-28 cells. Therefore, ACAK may be developed as a novel anticancer agent for the treatment of pancreatic cancer clinically. Citation Format: Weixiao An, Honglin Lai, Yangyyang Zhang, Xiukun Lin, Shousong Cao. Active components of Alpinia katsumadai-induced cell apoptosis and autophagy in human pancreatic cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3979.