Abstract 3837: Primary tumor cell lines derived from gastric PDX tumors displays differences in chemosensitivity

Abstract

Abstract Gastric cancer is a multifactorial and fatal disease with limited effective therapeutic options. Its overall mortality ranked third among cancer-related deaths worldwide. Patient-derived-xenograft (PDX) models have been widely used in the biomedical research. They recapitulate the genomic diversity/heterogeneity of patient tumors, maintain a lot of the characteristics of the original tumors and showed much better correlation with clinical outcome comparing to traditional cell line derived xenograft models. However, the relatively low throughput and time-consuming nature of PDX studies prevent them from being used in large scale screening or mechanistic studies. To facilitate drug development in gastric cancer, different cancer cell lines have been developed in our laboratory from gastric PDX tumors. We performed STR analyses to validate their identity and RNAseq data also showed high similarities to their parental PDX tumors. These primary gastric tumor cell lines displayed differences from each other in their chemo-sensitivities in vitro and in vivo. They also differ in their protein expression such as the HER-2 expression level. Pharmacology studies using different compounds showed similar drug responses of these cell lines in tissue culture, xenografts using these cell lines and the original PDX models. Our data demonstrated the high fidelity of these cell lines to their parental PDX tumors and serve as a bridge between the high-throughput drug screening and highly clinical relevant PDX models and will greatly facilitate the drug development in gastric cancer. Citation Format: Xiaomei Ge, Fulin Qiang, Yixin Zhang, Jibing Liu, Lei Yang, Yingying Qu, Fubo Xie, Xueting Li, Ying Gu, He Zhou. Primary tumor cell lines derived from gastric PDX tumors displays differences in chemosensitivity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3837. doi:10.1158/1538-7445.AM2017-3837